Review




Structured Review

Corning Life Sciences transwell® permeable supports
(A) Representative photos of <t>transwell</t> assay under different conditions. Scale bar: 200 μm. (B) Fluorescence images of H 2 O 2 stimulated 4T1 cells stained with DCF probe after pre-treatment with/without FCP NPs. Scale bar: 20 μm. (C) The level of IL-6 and TNF-α in cell culture supernatant incubated with/without LPS and pre-treated with/without FCP NPs. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs LPS group. (D) Study the expression of MMP-9 in 4T1 cells under different concentrations (unit: μg/mL) of FCP NPs by western blotting and (E) the corresponding quantitative analysis. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs control group.
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Images

1) Product Images from "Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis"

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis

Journal: Materials Today Bio

doi: 10.1016/j.mtbio.2025.102028

(A) Representative photos of transwell assay under different conditions. Scale bar: 200 μm. (B) Fluorescence images of H 2 O 2 stimulated 4T1 cells stained with DCF probe after pre-treatment with/without FCP NPs. Scale bar: 20 μm. (C) The level of IL-6 and TNF-α in cell culture supernatant incubated with/without LPS and pre-treated with/without FCP NPs. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs LPS group. (D) Study the expression of MMP-9 in 4T1 cells under different concentrations (unit: μg/mL) of FCP NPs by western blotting and (E) the corresponding quantitative analysis. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs control group.
Figure Legend Snippet: (A) Representative photos of transwell assay under different conditions. Scale bar: 200 μm. (B) Fluorescence images of H 2 O 2 stimulated 4T1 cells stained with DCF probe after pre-treatment with/without FCP NPs. Scale bar: 20 μm. (C) The level of IL-6 and TNF-α in cell culture supernatant incubated with/without LPS and pre-treated with/without FCP NPs. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs LPS group. (D) Study the expression of MMP-9 in 4T1 cells under different concentrations (unit: μg/mL) of FCP NPs by western blotting and (E) the corresponding quantitative analysis. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs control group.

Techniques Used: Transwell Assay, Fluorescence, Staining, Cell Culture, Incubation, Expressing, Western Blot, Control

Related Articles

Transwell Assay:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
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Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
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Migration:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
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Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Inverted Microscopy:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Fluorescence:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Staining:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Cell Culture:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Incubation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Expressing:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Western Blot:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Control:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

In Situ:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

In Vitro:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Membrane:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Confocal Microscopy:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Isolation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Flow Cytometry:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Transfection:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Pore Size:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Co-Culture Assay:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Quantitative RT-PCR:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Cell Fractionation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Knockdown:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Over Expression:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Cell Counting:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Injection:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Standard Deviation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Plasmid Preparation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Sequencing:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Binding Assay:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Luciferase:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

RNA Immunoprecipitation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Activation Assay:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Fractionation:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Phospho-proteomics:

Article Title: Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells.
Article Snippet: Objectives: Lipocalin 2 (LCN2) is a glycoprotein with multiple functions, including antimicrobial activity, inflammatory response modulation, and cell migration.. LCN2 is expressed in some cells, such as epithelial cells and neutrophils, and its levels are increased in inflammatory diseases.. This study investigated the presence of LCN2 receptor (24p3R) in cells around periodontal tissues and function of LCN2 in cells with a receptor to explore the role of LCN2 in periodontal diseases.

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis
Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus.
Article Snippet: Transwell® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).

Article Title: Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology
Article Snippet: The cell mixture was seeded onto Transwell® permeable supports (pore size: 0.4 μm, surface area: 1.12 cm 2 ; Corning, New York, NY, United States) at a density of 1 × 105 cells/cm 2 in 12-well cell culture multiwell plates (Corning).

Article Title: Active trans-corneal drug delivery with ocular adhesive micelles for efficient glaucoma therapy.
Article Snippet: Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea.. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of transcorneal drug delivery.. In this study, we demonstrate the potential of zwitterionic micelles composed of poly (2-(N-oxide-N,N-diethylamino)ethyl methacrylate)-block-poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery.

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1.
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [14].

Article Title: LINC02257 regulates malignant phenotypes of colorectal cancer via interacting with miR-1273g-3p and YB1
Article Snippet: Metastatic potential including invasive and migratory abilities was determined using the BD BiocoatTM Matrigel invasion chamber (BD Bioscience, San Jose, CA, USA) and Transwell® permeable supports (Corning, Rockville, MD, USA), respectively [ ].

Article Title: Polyzwitterion-branched polycholic acid nanocarriers based oral delivery insulin for long-term glucose and metabolic regulation in diabetes mellitus
Article Snippet: Transwell ® Permeable Supports (0.4 μm, Corning, USA), O.C.T gel (Tissue-Tek, Torrance, CA, USA).



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90
Corning Life Sciences transwell® permeable support
Photomicrographs show the effect of SGS on the <t>Transwell</t> migration of HK-2 cells, stained with toluidine blue O at 24 h, with accompanying graphs quantifying the number of migrated cells. Data are presented as mean ± SEM (n=3) from three independent experiments. * P<0.05 vs. normal control; # P<0.05 vs. NaOX control. Scale bar, 500 µm. SGS, sulfated galactan with increased sulfation; NaOX, sodium oxalate; Control, no treatment; NaOX control, treated with 1.25 mmol/l of NaOX; 100-SGS + NaOX, treated with 100 µg/ml of SGS combined with 1.25 mmol/l of NaOX; 1000-SGS + NaOX, treated with 1,000 µg/ml of SGS combined with 1.25 mmol/l of NaOX; Cystone + NaOX, treated with 100 µg/ml of Cystone combined with 1.25 mmol/l of NaOX.
Transwell® Permeable Support, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell%C2%AE+permeable+supports/96+well+plates/us12365863-756-12-12
Average 90 stars, based on 1 article reviews
transwell® permeable support - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Corning Life Sciences transwell permeable supports 3422
(A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in <t>transwell</t> migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Transwell Permeable Supports 3422, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell%C2%AE+permeable+supports/96+well+plates/bio_rxiv__2025__07__15__664801-134-5-9
Average 90 stars, based on 1 article reviews
transwell permeable supports 3422 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Corning Life Sciences hts-96 transwell permeable support chamber with pores
(A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in <t>transwell</t> migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
Hts 96 Transwell Permeable Support Chamber With Pores, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell%C2%AE+permeable+supports/hts+96+well+transwells/pmc12267471-160-18-19
Average 90 stars, based on 1 article reviews
hts-96 transwell permeable support chamber with pores - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


(A) Representative photos of transwell assay under different conditions. Scale bar: 200 μm. (B) Fluorescence images of H 2 O 2 stimulated 4T1 cells stained with DCF probe after pre-treatment with/without FCP NPs. Scale bar: 20 μm. (C) The level of IL-6 and TNF-α in cell culture supernatant incubated with/without LPS and pre-treated with/without FCP NPs. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs LPS group. (D) Study the expression of MMP-9 in 4T1 cells under different concentrations (unit: μg/mL) of FCP NPs by western blotting and (E) the corresponding quantitative analysis. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs control group.

Journal: Materials Today Bio

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis

doi: 10.1016/j.mtbio.2025.102028

Figure Lengend Snippet: (A) Representative photos of transwell assay under different conditions. Scale bar: 200 μm. (B) Fluorescence images of H 2 O 2 stimulated 4T1 cells stained with DCF probe after pre-treatment with/without FCP NPs. Scale bar: 20 μm. (C) The level of IL-6 and TNF-α in cell culture supernatant incubated with/without LPS and pre-treated with/without FCP NPs. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs LPS group. (D) Study the expression of MMP-9 in 4T1 cells under different concentrations (unit: μg/mL) of FCP NPs by western blotting and (E) the corresponding quantitative analysis. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs control group.

Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Techniques: Transwell Assay, Fluorescence, Staining, Cell Culture, Incubation, Expressing, Western Blot, Control

Photomicrographs show the effect of SGS on the Transwell migration of HK-2 cells, stained with toluidine blue O at 24 h, with accompanying graphs quantifying the number of migrated cells. Data are presented as mean ± SEM (n=3) from three independent experiments. * P<0.05 vs. normal control; # P<0.05 vs. NaOX control. Scale bar, 500 µm. SGS, sulfated galactan with increased sulfation; NaOX, sodium oxalate; Control, no treatment; NaOX control, treated with 1.25 mmol/l of NaOX; 100-SGS + NaOX, treated with 100 µg/ml of SGS combined with 1.25 mmol/l of NaOX; 1000-SGS + NaOX, treated with 1,000 µg/ml of SGS combined with 1.25 mmol/l of NaOX; Cystone + NaOX, treated with 100 µg/ml of Cystone combined with 1.25 mmol/l of NaOX.

Journal: Biomedical Reports

Article Title: Effect of Gracilaria fisheri sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate-induced HK-2 cell injury

doi: 10.3892/br.2025.2001

Figure Lengend Snippet: Photomicrographs show the effect of SGS on the Transwell migration of HK-2 cells, stained with toluidine blue O at 24 h, with accompanying graphs quantifying the number of migrated cells. Data are presented as mean ± SEM (n=3) from three independent experiments. * P<0.05 vs. normal control; # P<0.05 vs. NaOX control. Scale bar, 500 µm. SGS, sulfated galactan with increased sulfation; NaOX, sodium oxalate; Control, no treatment; NaOX control, treated with 1.25 mmol/l of NaOX; 100-SGS + NaOX, treated with 100 µg/ml of SGS combined with 1.25 mmol/l of NaOX; 1000-SGS + NaOX, treated with 1,000 µg/ml of SGS combined with 1.25 mmol/l of NaOX; Cystone + NaOX, treated with 100 µg/ml of Cystone combined with 1.25 mmol/l of NaOX.

Article Snippet: Transwell permeable supports (6.5 mm inserts, 24-well plates) were purchased from Corning Life Sciences.

Techniques: Migration, Staining, Control

(A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in transwell migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Journal: bioRxiv

Article Title: Canonical microRNA loss drives tumor development implicating therapeutic efficacy of enoxacin in angiosarcoma

doi: 10.1101/2025.07.15.664801

Figure Lengend Snippet: (A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in transwell migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Transwell assays were performed with transwell permeable supports (3422, Corning, Glendale, AZ, USA).

Techniques: Activity Assay, Migration